ZFIN ID: ZDB-LAB-110209-1
JY Chen Lab
PI/Director: Chen, Jyh-Yih
Contact Person: Chen, Jyh-Yih
Email: zoocjy@gate.sinica.edu.tw
URL: http://icob.sinica.edu.tw/en/staff_info.aspx?memberID=18
Address: Laboratory of Marine Molecular Biology and Biotechnology Institute of Zoology Academia Sinica 23-10 Dahuen Rd. Jiaushi Ilan 262 Taiwan
Country: Taiwan
Phone: 886-920802111
Fax: 886-39883197
Line Designation: asj

Show all 11 genomic features

When a fragment of genomic DNA is introduced into a mammalian cell, it can locate and recombine with endogenous homologous sequences. This type of homologous recombination is known as gene targeting by operating embryonic stem cells. As an alternative to embryonic stem cells, cultured somatic cells offer the possibility of producing cloned aquatic animals with targeted genetic manipulations by nuclear transfer. Attempts to produce cloned animals for commercial purposes have been made in cattle, sheep, rabbit, pig, and zebrafish, but were not successful in commercial aquatic animals. Our laboratory is focusing on platform technology development and applications to aquaculture.

1. Development of Site-specific Recombination in Fish Cells and Zebrafish
Recent advances using the Cre-loxP and Flp-FRT systems have now made it possible to generate “clean” germline mutations following a single gene targeting event, as well as to activate and inactivate genes in a condi- tional manner in living mice. These techniques have not yet been applied to fish systems. The techniques not only can target gene mutations which are induced in spatially and temporally restricted fashions, but lineage tracers can also be activated in specific progenitor populations to chart cell fates directly in wildtype and mutant animals. We have chosen two targeting genes: zebrafish IGFBP-2 (chromosome 22) and IGFBP-3. These two genes span around 20 kb in the zebrafish genome. We then studied the features of IGFBP-2 and IGFBP-3 promoter functions, and we now know the expression types in embryos driven by the GFP gene. The next step will be targeting these two genes in zebrafish cells, then carrying out nuclear transfer to study the knockout gene function in zebrafish system.

2. Application of Antimicrobial Peptide to Aquatic Diseases Resistance in Shrimp and Fish
In recent years, hundreds of naturally occurring peptide antibiotics have been discovered based on their ability to inhibit the growth of microbial pathogens. These antimicrobial peptides (AMPs) participate in the innate immune response by providing a rapid first-line defense against infection. To address the need for new therapies to combat resistant organisms, we are refocusing the discovery efforts on developing novel agents with new mechanisms of action by a knock-in technique. The hope is that with improvements in rapidly emerging technologies including gene transfer technology and transgenic aquatic animals, pathogen challenge will occur. These technologies should aid in the identification of novel AMPs as drug targets and compounds with unique mechanisms of action other than those currently provided by traditional antibiotics. We are investigating the roles of AMPs and interferon in aquatic animals using a transgenic approach.

3. Developing Biosafety Technology of Genetically Modified Aquatic Organisms by Site-specific Recombination
We cannot seek permission to market transgenic fish; the main reason is the lack of a field test research area and because no basic research technology has been developed to evaluate their biosafety. Sterility is a necessary adjunct to the exploitation of transgenic fish unless completely secure land-locked facilities are available. In addition, sterility is an important parameter in its own right, even aside from its use for containment of genetically modified fish. The problems posed by the escape of transgenic fish from sea cages and their interbreeding with wild populations in adjacent rivers are well known, and problems with different farmed fish species could at least be partially reduced by the use of sterile fish. Sterility in fish can be achieved by two different routes, namely ploidy manipulation to produce sterile triploids, or the use of transgenesis to achieve gene “knockout” or gene “knockdown” by an RNAi approach with the zebrafish GtH gene.


Ting, C.H., Chen, Y.C., Chen, J.Y. (2018) Nile tilapia fry fed on antimicrobial peptide Epinecidin-1-expressing Artemia cyst exhibit enhanced immunity against acute bacterial infection. Fish & shellfish immunology. 81:37-48
Pan, C.Y., Liu, Y.H., Gong, H.Y., Chen, J.Y. (2017) Transcriptome analysis of the effect of polyunsaturated fatty acids against Vibrio vulnificus infection in Oreochromis niloticus. Fish & shellfish immunology. 62:153-163
Jheng, Y.H., Lee, L.H., Ting, C.H., Pan, C.Y., Hui, C.F., Chen, J.Y. (2015) Zebrafish fed on recombinant Artemia expressing epinecidin-1 exhibit increased survival and altered expression of immunomodulatory genes upon Vibrio vulnificus infection. Fish & shellfish immunology. 42:1-15
Wang, Y.D., Peng, K.C., Wu, J.L., Chen, J.Y. (2014) Transgenic expression of salmon delta-5 and delta-6 desaturase in zebrafish muscle inhibits the growth of Vibrio alginolyticus and affects fish immunomodulatory activity. Fish & shellfish immunology. 39(2):223-30
Lin, H.J., Lee, S.H., Wu, J.L., Duann, Y.F., and Chen, J.Y. (2013) Development of Cre-loxP technology in zebrafish to study the regulation of fish reproduction. Fish physiology and biochemistry. 39(6):1525-39
Lee, S.H., Peng, K.C., Lee, L.H., Pan, C.Y., Hour, A.L., Her, G.M., Hui, C.F., and Chen, J.Y. (2013) Characterization of tilapia (Oreochromis niloticus) viperin expression, and inhibition of bacterial growth and modulation of immune-related gene expression by electrotransfer of viperin DNA into zebrafish muscle. Veterinary Immunology and Immunopathology. 151(3-4):217-228
Huang, T.C., and Chen, J.Y. (2013) Proteomic and functional analysis of zebrafish after administration of antimicrobial peptide epinecidin-1. Fish & shellfish immunology. 34(2):593-598
Pan, C.Y., Huang, T.C., Wang, Y.D., Yeh, Y.C., Hui, C.F., and Chen, J.Y. (2012) Oral administration of recombinant epinecidin-1 protected grouper (Epinephelus coioides) and zebrafish (Danio rerio) from Vibrio vulnificus infection and enhanced immune-related gene expressions. Fish & shellfish immunology. 32(6):947-957
Pan, C.Y., Wu, J.L., Hui, C.F., Lin, C.H., and Chen, J.Y. (2011) Insights into the antibacterial and immunomodulatory functions of the antimicrobial peptide, epinecidin-1, against Vibrio vulnificus infection in zebrafish. Fish & shellfish immunology. 31(6):1019-25
Pan, C.Y., Peng, K.C., Lin, C.H., and Chen, J.Y. (2011) Transgenic expression of tilapia hepcidin 1-5 and shrimp chelonianin in zebrafish and their resistance to bacterial pathogens. Fish & shellfish immunology. 31(2):275-85
Chen, J.Y., and Chiou, M.J. (2010) Molecular cloning and functional analysis of the zebrafish luteinizing hormone beta subunit (LH) promoter. Fish physiology and biochemistry. 36(4):1253-1262
Chen, L.C., Wu, J.L., Shiau, C.Y., and Chen, J.Y. (2010) Organization and promoter analysis of the zebrafish (Danio rerio) chemokine gene (CXC-64) promoter. Fish physiology and biochemistry. 36(3):511-521
Peng, K.C., Pan, C.Y., Chou, H.N., and Chen, J.Y. (2010) Using an improved Tol2 transposon system to produce transgenic zebrafish with epinecidin-1 which enhanced resistance to bacterial infection. Fish & shellfish immunology. 28(5-6):905-917
Chen, J.Y., Chiou, M.J., Chen, L.K., and Wu, J.L. (2010) Molecular cloning and functional analysis of the zebrafish follicle-stimulating hormone (FSH)beta promoter. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 155(2):155-163
Lin, S.B., Fan, T.W., Wu, J.L., Hui, C.F., and Chen, J.Y. (2009) Immune response and inhibition of bacterial growth by electrotransfer of plasmid DNA containing the antimicrobial peptide, epinecidin-1, into zebrafish muscle. Fish & shellfish immunology. 26(3):451-458
Chen, L.C., Chen, J.Y., Hour, A.L., Shiau, C.Y., Hui, C.F., and Wu, J.L. (2008) Molecular cloning and functional analysis of zebrafish (Danio rerio) chemokine genes. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 151(4):400-409
Pan, C.Y., Chen, J.Y., Ni, I.H., Wu, J.L., and Kuo, C.M. (2008) Organization and promoter analysis of the grouper (Epinephelus coioides) epinecidin-1 gene. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 150(4):358-367
Huang, W.T., Hsieh, J.C., Chiou, M.J., Chen, J.Y., Wu, J.L., and Kuo, C.M. (2008) Application of RNAi Technology to the Inhibition of Zebrafish GtHalpha, FSHbeta, and LHbeta Expression and to Functional Analyses. Zoological science. 25(6):614-621
Chiou, M.J., Wang, Y.D., Kuo, C.M., Chen, J.C., and Chen, J.Y. (2007) Functional Analysis of Mitogen-Activated Protein Kinase-3 (MAPK3) and Its Regulation of the Promoter Region in Zebrafish. DNA and cell biology. 26(11):781-790
Chou, M.J., Chen, J.C., Chen, J.Y., Gong, H.Y., Li, L.T., Huang, T.C., Wu, J.L., and Kuo, C.M. (2006) Isolation and characterization of the zebrafish Danio rerio insulin-like growth factor binding protein-3 promoter region. Fisheries science : FS. 74:153-166
Chiou, M.J., Chao, T.T., Wu, J.L., Kuo, C.M., and Chen, J.Y. (2006) The physiological role of CTGF/CCN2 in zebrafish notochond development and biological analysis of the proximal promoter region. Biochemical and Biophysical Research Communications. 349(2):750-758
Chen, J.Y., You, Y.K., Chen, J.C., Huang, T.C., and Kuo, C.M. (2005) Organization and Promoter Analysis of the Zebrafish (Danio rerio) Interferon Gene. DNA and cell biology. 24(10):641-650
Chen, J.Y., Chou, M.J., Gong, H.Y., Huang, T.C., Wu, J.L., and Kuo, C.M. (2005) Cloning and Biological Analysis of the Zebrafish (Danio rerio) Insulin-Like Growth Factor Binding Protein-2 Proximal Promoter Region. DNA and cell biology. 24(3):199-208
Chen, J.Y., Chen, J.C., Huang, W.T., Liu, C.W., Hui, C.F., Chen, T.T., and Wu, J.L. (2004) Molecular cloning and tissue-specific, developmental-stage-specific, and hormonal regulation of IGFBP3 gene in zebrafish. Marine biotechnology (New York, N.Y.). 6(1):1-7
Chen, J.Y., Chen, J.C., and Wu, J.L. (2003) Molecular cloning and functional analysis of zebrafish high-density lipoprotein-binding protein. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 136(1):117-130
Chen, J.-Y., Chang, B.-E., Chen, Y.-H., Lin, C.J.F., Wu, J.-L., and Kuo, C.-M. (2001) Molecular Cloning, Developmental Expression, and Hormonal Regulation of Zebrafish (Danio rerio) β Crystallin B1, a Member of the Superfamily of β Crystallin Proteins. Biochemical and Biophysical Research Communications. 285(1):105-110
Chen, J.-Y., Yang, J.-Y., Wang, J.-I., and Wu, J.-L. (2001) Introduction of green fluorescent protein and Lac-Z gene into zebrafish (Danio rerio) egg by microinjection and particle gun. J. Fish. Soc. Taiwan. 28(2):91-103