ZFIN ID: ZDB-LAB-100630-2
Huisken Lab
PI/Director: Huisken, Jan
Contact Person: Huisken, Jan
Email: huisken@mpi-cbg.de
URL: http://www.mpi-cbg.de/huisken
Address: Pfotenhauerstr. 108 Dresden 01307 Germany
Country: Germany
Phone: +49-351-210-2487
Fax: +49-351-210-1209
Line Designation: cbg


GENOMIC FEATURES ORIGINATING FROM THIS LAB
Show all 2 genomic features


STATEMENT OF RESEARCH INTERESTS
The overall goal of the newly formed lab is the systematic study of developmental processes in living organisms by noninvasive biomedical imaging techniques such as optical microscopy. Of primary interest is the investigation of organogenesis in zebrafish with special emphasis on the function and morphogenesis of the cardiovascular system. We develop novel quantitative microscopy tools and experimental strategies to understand and describe tissue dynamics on a cellular level. High-speed fluorescence microscopy is the primary tool to capture the dynamics of a heartbeat and the fate of single cells during organogenesis. We address all experimental steps from innovative transgenic lines and microscope development to systematic image processing.

One of the technologies that are being developed is selective plane illumination microscopy (SPIM). This fluorescence microscopy technique uses a focused light-sheet to illuminate the specimen from the side. SPIM achieves excellent resolution at high penetration depths while being minimally invasive at the same time. SPIM offers a number of advantages over established techniques such as strongly reduced photo-bleaching, high dynamic range, and high acquisition speed. The translucent nature of the zebrafish embryo allows for observation of the heart and the whole cardiovascular system in the living animal. Optical manipulation tools like photo-convertible fluorophores, photo-activatable ion-channels, and laser nanosurgery are used in addition to image acquisition.

The lab takes a truly interdisciplinary approach. Scientists with various backgrounds collaborate and develop novel experimental strategies to achieve a common goal.


LAB MEMBERS
Hombach, Sonja Post-Doc


ZEBRAFISH PUBLICATIONS OF LAB MEMBERS
Shah, G., Thierbach, K., Schmid, B., Waschke, J., Reade, A., Hlawitschka, M., Roeder, I., Scherf, N., Huisken, J. (2019) Multi-scale imaging and analysis identify pan-embryo cell dynamics of germlayer formation in zebrafish. Nature communications. 10:5753
Rosowski, E.E., He, J., Huisken, J., Keller, N.P., Huttenlocher, A. (2019) Efficacy of voriconazole against A. fumigatus infection depends on host immune function. Antimicrobial Agents and Chemotherapy. 64(2):
Prummel, K.D., Hess, C., Nieuwenhuize, S., Parker, H.J., Rogers, K.W., Kozmikova, I., Racioppi, C., Brombacher, E.C., Czarkwiani, A., Knapp, D., Burger, S., Chiavacci, E., Shah, G., Burger, A., Huisken, J., Yun, M.H., Christiaen, L., Kozmik, Z., Müller, P., Bronner, M., Krumlauf, R., Mosimann, C. (2019) A conserved regulatory program initiates lateral plate mesoderm emergence across chordates. Nature communications. 10:3857
Kugler, E.C., van Lessen, M., Daetwyler, S., Chhabria, K., Savage, A.M., Silva, V., Plant, K., MacDonald, R.B., Huisken, J., Wilkinson, R.N., Schulte-Merker, S., Armitage, P., Chico, T.J. (2019) Cerebrovascular endothelial cells form transient Notch-dependent cystic structures in zebrafish. EMBO reports. 20:e47047
Chrispijn, N.D., Elurbe, D.M., Mickoleit, M., Aben, M., de Bakker, D.E.M., Andralojc, K.M., Huisken, J., Bakkers, J., Kamminga, L.M. (2019) Loss of the Polycomb group protein Rnf2 results in derepression of tbx-transcription factors and defects in embryonic and cardiac development. Scientific Reports. 9:4327
Daetwyler, S., Günther, U., Modes, C.D., Harrington, K., Huisken, J. (2019) Multi-sample SPIM image acquisition, processing and analysis of vascular growth in zebrafish. Development (Cambridge, England). 146(6):
Berndt, F., Shah, G., Power, R.M., Brugués, J., Huisken, J. (2018) Dynamic and non-contact 3D sample rotation for microscopy. Nature communications. 9:5025
Chhabria, K., Plant, K., Bandmann, O., Wilkinson, R.N., Martin, C., Kugler, E., Armitage, P.A., Santoscoy, P.L., Cunliffe, V.T., Huisken, J., McGown, A., Ramesh, T., Chico, T.J., Howarth, C. (2018) The effect of hyperglycemia on neurovascular coupling and cerebrovascular patterning in zebrafish. Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism. 40(2):298-313
Attardi, A., Fulton, T., Florescu, M., Shah, G., Muresan, L., Lenz, M.O., Lancaster, C., Huisken, J., van Oudenaarden, A., Steventon, B. (2018) Neuromesodermal progenitors are a conserved source of spinal cord with divergent growth dynamics. Development (Cambridge, England). 145(21):
Power, R.M., Huisken, J. (2018) Adaptable, illumination patterning light sheet microscopy. Scientific Reports. 8:9615
Felker, A., Prummel, K.D., Merks, A.M., Mickoleit, M., Brombacher, E.C., Huisken, J., Panáková, D., Mosimann, C. (2018) Continuous addition of progenitors forms the cardiac ventricle in zebrafish. Nature communications. 9:2001
Weber, M., Scherf, N., Meyer, A.M., Panáková, D., Kohl, P., Huisken, J. (2017) Cell-accurate optical mapping across the entire developing heart. eLIFE. 6
Jahr, W., Schmid, B., Weber, M., Huisken, J. (2016) eduSPIM: Light Sheet Microscopy in the Museum. PLoS One. 11:e0161402
Weber, M., Huisken, J. (2015) In vivo imaging of cardiac development and function in zebrafish using light sheet microscopy. Swiss medical weekly. 145:w14227
Jahr, W., Schmid, B., Schmied, C., Fahrbach, F.O., Huisken, J. (2015) Hyperspectral light sheet microscopy. Nature communications. 6:7990
Lenard, A., Daetwyler, S., Betz, C., Ellertsdottir, E., Belting, H.G., Huisken, J., Affolter, M. (2015) Endothelial Cell Self-fusion during Vascular Pruning. PLoS Biology. 13(4):e1002126
Bassi, A., Schmid, B., Huisken, J. (2015) Optical tomography complements light sheet microscopy for in toto imaging of zebrafish development. Development (Cambridge, England). 142(5):1016-20
Renz, M., Otten, C., Faurobert, E., Rudolph, F., Zhu, Y., Boulday, G., Duchene, J., Mickoleit, M., Dietrich, A.C., Ramspacher, C., Steed, E., Manet-Dupé, S., Benz, A., Hassel, D., Vermot, J., Huisken, J., Tournier-Lasserve, E., Felbor, U., Sure, U., Albiges-Rizo, C., Abdelilah-Seyfried, S. (2015) Regulation of β1 Integrin-Klf2-Mediated Angiogenesis by CCM Proteins. Developmental Cell. 32:181-190
Junker, J.P., Noël, E.S., Guryev, V., Peterson, K.A., Shah, G., Huisken, J., McMahon, A.P., Berezikov, E., Bakkers, J., van Oudenaarden, A. (2014) Genome-wide RNA Tomography in the Zebrafish Embryo. Cell. 159:662-75
Mickoleit, M., Schmid, B., Weber, M., Fahrbach, F.O., Hombach, S., Reischauer, S., Huisken, J. (2014) High-resolution reconstruction of the beating zebrafish heart. Nature Methods. 11(9):919-22
Alvers, A.L., Ryan, S., Scherz, P.J., Huisken, J., and Bagnat, M. (2014) Single continuous lumen formation in the zebrafish gut is mediated by smoothened-dependent tissue remodeling. Development (Cambridge, England). 141(5):1110-1119
Weber, M., Mickoleit, M., Huisken, J. (2014) Multilayer mounting for long-term light sheet microscopy of zebrafish. Journal of visualized experiments : JoVE. (84):e51119
Weber, M., Mickoleit, M., Huisken, J. (2014) Light sheet microscopy. Methods in cell biology. 123C:193-215
Fahrbach, F.O., Voigt, F.F., Schmid, B., Helmchen, F., and Huisken, J. (2013) Rapid 3D light-sheet microscopy with a tunable lens. Optics express. 21(18):21010-21026
Schmid, B., Shah, G., Scherf, N., Weber, M., Thierbach, K., Campos, C.P., Roeder, I., Aanstad, P., and Huisken, J. (2013) High-speed panoramic light-sheet microscopy reveals global endodermal cell dynamics. Nature communications. 4:2207
Opitz, R., Maquet, E., Huisken, J., Antonica, F., Trubiroha, A., Pottier, G., Janssens, V., and Costagliola, S. (2012) Transgenic zebrafish illuminate the dynamics of thyroid morphogenesis and its relationship to cardiovascular development. Developmental Biology. 372(2):203-216
Mellman, K., Huisken, J., Dinsmore, C., Hoppe, C., and Stainier, D.Y. (2012) Fibrillin-2b regulates endocardial morphogenesis in zebrafish. Developmental Biology. 372(1):111-119
Kaufmann, A., Mickoleit, M., Weber, M., and Huisken, J. (2012) Multilayer mounting enables long-term imaging of zebrafish development in a light sheet microscope. Development (Cambridge, England). 139(17):3242-3247
Kirchmaier, B.C., Poon, K.L., Schwerte, T., Huisken, J., Winkler, C., Jungblut, B., Stainier, D.Y., and Brand, T. (2012) The Popeye domain containing 2 (popdc2) gene in zebrafish is required for heart and skeletal muscle development. Developmental Biology. 363(2):438-450
Weber, M., and Huisken, J. (2011) Light sheet microscopy for real-time developmental biology. Current opinion in genetics & development. 21(5):566-72
Arrenberg, A.B., Stainier, D.Y., Baier, H., and Huisken, J. (2010) Optogenetic control of cardiac function. Science (New York, N.Y.). 330(6006):971-974
Liu, J., Bressan, M., Hassel, D., Huisken, J., Staudt, D., Kikuchi, K., Poss, K.D., Mikawa, T., and Stainier, D.Y. (2010) A dual role for ErbB2 signaling in cardiac trabeculation. Development (Cambridge, England). 137(22):3867-3875
Bagnat, M., Navis, A., Herbstreith, S., Brand-Arzamendi, K., Curado, S., Gabriel, S., Mostov, K., Huisken, J., and Stainier, D.Y. (2010) Cse1l Is a Negative Regulator of CFTR-Dependent Fluid Secretion. Current biology : CB. 20(20):1840-1845
Chi, N.C., Shaw, R.M., Jungblut, B., Huisken, J., Ferrer, T., Arnaout, R., Scott, I., Beis, D., Xiao, T., Baier, H., Jan, L.Y., Tristani-Firouzi, M., and Stainier, D.Y. (2008) Genetic and Physiologic Dissection of the Vertebrate Cardiac Conduction System. PLoS Biology. 6(5):e109
Arnaout, R., Ferrer, T., Huisken, J., Spitzer, K., Stainier, D.Y., Tristani-Firouzi, M., and Chi, N.C. (2007) Zebrafish model for human long QT syndrome. Proceedings of the National Academy of Sciences of the United States of America. 104(27):11316-11321