ZFIN ID: ZDB-LAB-080519-1
Biemar Lab
PI/Director: Biemar, Frédéric
Co-PI / Senior
Researcher:
Georlette, Daphne
Contact Person: Georlette, Daphne
Email: daph@temple.edu
URL: http://www.openwetware.org/wiki/Biemar
Address: Department of Biology Temple University 439 Biology Life Sciences Building 1900 North 12th Street Philadelphia, PA 19122
Country: United States
Phone: 215-204-1925
Fax: 215-204-6646
Line Designation: tpl


GENOMIC FEATURES ORIGINATING FROM THIS LAB No data available


STATEMENT OF RESEARCH INTERESTS
We use the zebrafish as a model organism to study pancreas organogenesis.

Initial studies showed that, albeit some minor differences, the essential components (transcription factors and signaling molecules) involved in pancreas organogenesis in zebrafish and mammals are conserved. Nonetheless, the gene network underlying pancreas development in zebrafish and mammals is incomplete because mainly based on epistatic relationships inferred from knockout phenotypes. In addition, recent surveys of the embryonic expression of microRNAs during zebrafish development revealed at least two that appear to be pancreas-specific. The function of these miRNAs in pancreas formation is unknown.

What are the genes directly regulated by some of the major transcription factors involved in pancreas organogenesis (i.e. Ptf1, Pdx, Hb9, Isl1, Pax6, Nk2.2,...)? What are the target cis-regulatory modules bound by those factors? Are target genes regulated by these factors combinatorially? What is the role of microRNAs expressed in the pancreatic anlage during zebrafish embryogenesis? We address those questions using a combination of ChiP-seq and SELEX assays, classical deletion studies, comparative genomics and other bioinformatics methods, as well as reverse genetics.


LAB MEMBERS


ZEBRAFISH PUBLICATIONS OF LAB MEMBERS
Balciuniene, J., Nagelberg, D., Walsh, K., Camerota, D., Georlette, D., Biemar, F., Bellipanni, G., and Balciunas, D. (2013) Efficient disruption of Zebrafish genes using a Gal4-containing gene trap. BMC Genomics. 14(1):619
Verbruggen, V., Ek, O., Georlette, D., Delporte, F., Von Berg, V., Detry, N., Biemar, F., Coutinho, P., Martial, J.A., Voz, M.L., Manfroid, I., and Peers, B. (2010) The Pax6b homeodomain is dispensable for pancreatic endocrine cell differentiation in zebrafish. The Journal of biological chemistry. 285(18):13863-13873
Delporte, F.M., Pasque, V., Devos, N., Manfroid, I., Voz, M.L., Motte, P., Biemar, F., Martial, J.A., and Peers, B. (2008) Expression of zebrafish pax6b in pancreas is regulated by two enhancers containing highly conserved cis-elements bound by PDX1, PBX and PREP factors. BMC Developmental Biology. 8:53
Zecchin, E., Filippi, A., Biemar, F., Tiso, N., Pauls, S., Ellertsdottir, E., Gnugge, L., Bortolussi, M., Driever, W., and Argenton, F. (2007) Distinct delta and jagged genes control sequential segregation of pancreatic cell types from precursor pools in zebrafish. Developmental Biology. 301(1):192-204
Ellertsdottir, E., Ganz, J., Durr, K., Loges, N., Biemar, F., Seifert, F., Ettl, A.K., Kramer-Zucker, A.K., Nitschke, R., and Driever, W. (2006) A mutation in the zebrafish Na,K-ATPase subunit atp1a1a.1 provides genetic evidence that the sodium potassium pump contributes to left-right asymmetry downstream or in parallel to nodal flow. Developmental dynamics : an official publication of the American Association of Anatomists. 235(7):1794-1808
Mavropoulos, A., Devos, N., Biemar, F., Zecchin, E., Argenton, F., Edlund, H., Motte, P., Martial, J.A., and Peers, B. (2005) sox4b is a key player of pancreatic alpha cell differentiation in zebrafish. Developmental Biology. 285(1):211-223
de Cat, B., Muyldermans, S.Y., Coomans, C., Degeest, G., Vanderschueren, B., Creemers, J., Biemar, F., Peers, B., and David, G. (2003) Processing by proprotein convertases is required for glypican-3 modulation of cell survival, Wnt signaling, and gastrulation movements. The Journal of cell biology. 163(3):625-635
Biemar, F., Baraldi, F., Devos, N., Holzschuh, J., Martial, J.A., Driever, W., and Peers, B. (2002) Analysis of meis genes expression in zebrafish suggests a role in the development of organs derived from the endoderm. Developmental Biology. 247(2):495
Devos, N., Deflorian, G., Biemar, F., Bortolussi, M., Martial, J.A., Peers, B., and Argenton, F. (2002) Differential expression of two somatostatin genes during zebrafish embryonic development. Mechanisms of Development. 115(1-2):133-137
Biemar, F., Devos, N., Martial, J.A., Driever, W., and Peers, B. (2001) Cloning and expression of the TALE superclass homeobox Meis2 gene during zebrafish embryonic development. Mechanisms of Development. 109(2):427-431
Molina, A., Iyengar, A., Marins, L.F., Biemar, F., Hanley, S., Maclean, N., Smith, T.J., Martial, J.A., and Muller, M. (2001) Gene structure and promoter function of a teleost ribosomal protein: a tilapia (Oreochromis mossambicus) L18 gene. BBA Gene Structure and Expression. 1520(3):195-202
Biemar, F., Argenton, F., Schmidtke, R., Epperlein, S., Peers, B., and Driever, W. (2001) Pancreas development in zebrafish: early dispersed appearance of endocrine hormone expressing cells and their convergence to form the definitive islet. Developmental Biology. 230(2):189-203
Molina, A., Biemar, F., Müller, F., Iyengar, A., Prunet, P., Maclean, N., Martial, J.A., and Muller, M. (2000) Cloning and expression analysis of an inducible HSP70 gene from tilapia fish. FEBS letters. 474(1):5-10