ZFIN ID: ZDB-LAB-001113-2
Nicolson Lab
PI/Director: Nicolson, Teresa
Contact Person: Nicolson, Teresa
Email: nicolson@ohsu.edu
URL: http://www.ohsu.edu/xd/research/centers-institutes/vollum/faculty/faculty-profile.cfm?facultyID=269
Address: Oregon Hearing and Research Center and Vollum Institute Oregon Health & Science University 3181 SW Sam Jackson Park Road Portland, Oregon 97239 USA
Phone: (503) 494-2928
Fax: (503) 494-9612
Line Designation: vo


Defects in sensory hair cells are thought to account for most forms of hereditary deafness. We aim to identify the components involved in hair-cell function by using a genetic approach. By screening over 7000 genomes (Tuebingen Screens I & II), we have identified a total of 24 genes that are required for balance and hearing in zebrafish. These circler mutants are analogous to the mouse shaker-waltzer mutants and can serve as alternative models for the study of human deafness. We are currently developing new ways of analyzing our mutants such stably expressing hair-cell specific markers, creating behavioral tests for auditory and vestibular function, and measuring physiological responses in hair cells and acousticolateralis nerves.

Erickson, Tim Post-Doc Maeda, Reo Post-Doc Pacentine, Itallia Graduate Student
Hayashi, Lisa Fish Facility Staff Gibson, Daniel Technical Staff

Sheets, L., Hagen, M.W., Nicolson, T. (2014) Characterization of Ribeye Subunits in Zebrafish Hair Cells Reveals That Exogenous Ribeye B-Domain and CtBP1 Localize to the Basal Ends of Synaptic RibbonsPLoS One9:e107256.
Maeda, R., Kindt, K.S., Mo, W., Morgan, C.P., Erickson, T., Zhao, H., Clemens-Grisham, R., Barr-Gillespie, P.G., Nicolson, T. (2014) Tip-link protein protocadherin 15 interacts with transmembrane channel-like proteins TMC1 and TMC2Proc. Natl. Acad. Sci. USA111(35):12907-12.
Grisham, R.C., Kindt, K., Finger-Baier, K., Schmid, B., and Nicolson, T. (2013) Mutations in ap1b1 Cause Mistargeting of the Na(+)/K(+)-ATPase Pump in Sensory Hair CellsPLoS One8(4):e60866.
Kalueff, A.V., Gebhardt, M., Stewart, A.M., Cachat, J.M., Brimmer, M., Chawla, J.S., Craddock, C., Kyzar, E.J., Roth, A., Landsman, S., Gaikwad, S., Robinson, K., Baatrup, E., Tierney, K., Shamchuk, A., Norton, W., Miller, N., Nicolson, T., Braubach, O., Gilman, C.P., Pittman, J., Rosemberg, D.B., Gerlai, R., Echevarria, D., Lamb, E., Neuhauss, S.C., Weng, W., and Bally-Cuif, L. (2013) Towards a comprehensive catalog of zebrafish behavior 1.0 and beyondZebrafish10(1):70-86.
Sheets, L., Kindt, K.S., and Nicolson, T. (2012) Presynaptic CaV1.3 Channels Regulate Synaptic Ribbon Size and Are Required for Synaptic Maintenance in Sensory Hair CellsJ. Neurosci.32(48):17273-17286.
Obholzer, N., Swinburne, I.A., Schwab, E., Nechiporuk, A.V., Nicolson, T., and Megason, S.G. (2012) Rapid positional cloning of zebrafish mutations by linkage and homozygosity mapping using whole-genome sequencingDevelopment139(22):4280-4290.
Glover, G., Mueller, K.P., Söllner, C., Neuhauss, S.C., and Nicolson, T. (2012) The Usher gene cadherin 23 is expressed in the zebrafish brain and a subset of retinal amacrine cellsMol. Vis.18:2309-2322.
Einhorn, Z., Trapani, J.G., Liu, Q., and Nicolson, T. (2012) Rabconnectin3alpha promotes stable activity of the H+ pump on synaptic vesicles in hair cellsJ. Neurosci.32(32):11144-11156.
Kindt, K.S., Finch, G., and Nicolson, T. (2012) Kinocilia mediate mechanosensitivity in developing zebrafish hair cellsDev. Cell23(2):329-341.
Mo, W., and Nicolson, T. (2011) Both pre- and postsynaptic activity of nsf prevents degeneration of hair-cell synapsesPLoS One6(11):e27146.
Erickson, T., Pillay, L.M., and Waskiewicz, A.J. (2011) Zebrafish Tshz3b negatively regulates Hox function in the developing hindbrainGenesis49(9):725-42.
Sheets, L., Trapani, J.G., Mo, W., Obholzer, N., and Nicolson, T. (2011) Ribeye is required for presynaptic CaV1.3a channel localization and afferent innervation of sensory hair cellsDevelopment138(7):1309-1319.
Trapani, J.G., and Nicolson, T. (2011) Mechanism of spontaneous activity in afferent neurons of the zebrafish lateral-line organJ. Neurosci.31(5):1614-1623.
Trapani, J.G., and Nicolson, T. (2010) Chapter 8 - Physiological recordings from zebrafish lateral-line hair cells and afferent neuronsMeth. Cell. Biol.100:219-231.
Mo, W., Chen, F., Nechiporuk, A., and Nicolson, T. (2010) Quantification of vestibular-induced eye movements in zebrafish larvaeBMC Neurosci.11:110.
Wright, M.A., Mo, W., Nicolson, T., and Ribera, A.B. (2010) In vivo evidence for transdifferentiation of peripheral neuronsDevelopment137(18):3047-3056.
Trapani, J.G., Obholzer, N., Mo, W., Brockerhoff, S.E., and Nicolson, T. (2009) Synaptojanin1 is required for temporal fidelity of synaptic transmission in hair cellsPLoS Genet.5(5):e1000480.
Obholzer, N., Wolfson, S., Trapani, J.G., Mo, W., Nechiporuk, A., Busch-Nentwich, E., Seiler, C., Sidi, S., Söllner, C., Duncan, R.N., Boehland, A., and Nicolson, T. (2008) Vesicular glutamate transporter 3 is required for synaptic transmission in zebrafish hair cellsJ. Neurosci.28(9):2110-2118.
Geisler, R., Rauch, G.J., Geiger-Rudolph, S., Albrecht, A., van Bebber, F., Berger, A., Busch-Nentwich, E., Dahm, R., Dekens, M.P., Dooley, C., Elli, A.F.,Gehring, I., Geiger, H., Geisler, M., Glaser, S., Holley, S., Huber, M., Kerr, A., Kirn, A., Knirsch, M., Konantz, M., Kuchler, A.M., Maderspacher, F., Neuhauss, S.C., Nicolson, T., Ober, E.A., Praeg, E., Ray, R., Rentzsch, B., Rick, J.M., Rief, E., Schauerte, H.E., Schepp, C.P., Schonberger, U., Schonthaler, H.B., Seiler, C., Sidi, S., Söllner, C., Wehner, A., Weiler, C., Nüsslein-Volhard, C. (2007) Large-scale mapping of mutations affecting zebrafish developmentBMC Genomics8(1):11.
Nicolson, T. (2005) The genetics of hearing and balance in zebrafishAnnu. Rev. Genet.39:9-22.
Söllner, C., and Nicolson, T. (2005) The Zebrafish as a Genetic Model to Study Otolith FormationBiomineralization (2nd ed.):229-242.
Seiler, C., Finger-Baier, K.C., Rinner, O., Makhankov, Y.V., Schwarz, H., Neuhauss, S.C., and Nicolson, T. (2005) Duplicated genes with split functions: independent roles of protocadherin15 orthologues in zebrafish hearing and visionDevelopment132(3):615-623.
Söllner, C., Schwarz, H., Geisler, R., and Nicolson, T. (2004) Mutated otopetrin 1 affects the genesis of otoliths and the localization of Starmaker in zebrafishDev. Genes Evol.214(12):582-590.
Seiler, C., Ben-David, O., Sidi, S., Hendrich, O., Rusch, A., Burnside, B., Avraham, K.B., and Nicolson, T. (2004) Myosin VI is required for structural integrity of the apical surface of sensory hair cells in zebrafishDev. Biol.272(2):328-338.
Paukert, M., Sidi, S., Russell, C., Siba, M., Wilson, S.W., Nicolson, T., and Grunder, S. (2004) A family of acid-sensing ion channels (ASICs) from the zebrafish: Widespread expression in the central nervous system suggests a conserved role in neuronal communicationJ. Biol. Chem.279(18):18783-18791.
Söllner, C., Rauch, G-J., Siemens, J., Geisler, R., Schuster, S.C., The Tübingen 2000 Screen Consortium, Müller, U., and Nicolson, T. (2004) Mutations in cadherin 23 affect tip links in zebrafish sensory hair cellsNature428(6986):955-959.
Sidi, S., Busch-Nentwich, E., Friedrich, R., Schoenberger, U., and Nicolson, T. (2004) gemini encodes a zebrafish L-type calcium channel that localizes at sensory hair cell ribbon synapsesJ. Neurosci.24(17):4213-4223.
Busch-Nentwich, E., Söllner, C., Roehl, H., and Nicolson, T. (2004) The deafness gene dfna5 is crucial for ugdh expression and HA production in the developing ear in zebrafishDevelopment131(4):943-951.
Söllner, C., Burghammer, M., Busch-Nentwich, E., Berger, J., Schwarz, H., Riekel, C., and Nicolson, T. (2003) Control of crystal size and lattice formation by starmaker in otolith biomineralizationScience302(5643):282-286.
Concha, M.L., Russell, C., Regan, J.C., Tawk, M., Sidi, S., Gilmour, D.T., Kapsimali, M., Sumoy, L., Goldstone, K., Amaya, E., Kimelman, D., Nicolson, T., Gründer, S., Gomperts, M., Clarke, J.D.W., and Wilson, S.W. (2003) Local tissue interactions across the dorsal midline of the forebrain establish CNS lateralityNeuron39(3):423-438.
Sidi, S., Friedrich, R.W., and Nicolson, T. (2003) NompC TRP channel required for vertebrate sensory hair cell mechanotransductionScience301(5629):96-99.
Ernest, S., Rauch, G.J., Haffter, P., Geisler, R., Petit, C., and Nicolson, T. (2000) Mariner is defective in myosin VIIA: a zebrafish model for human hereditary deafnessHum. Mol. Genet.9(14):2189-2196.
Nicolson, T., Ernest, S., Rauch, J., Petit, C., Söllner, C., Seiler, C., and Busch, E. (2000) Isolation of the genes responsible for hearing disorders in zebrafishEur. J. Neurosci.12:316.
Seiler, C. and Nicolson, T. (1999) Defective calmodulin-dependent rapid apical endocytosis in zebrafish sensory hair cell mutantsJ. Neurobiol.41(3):424-434.
Geisler, R., Rauch, G.J., Baier, H., van Bebber, F., Brobeta, L., Dekens, M.P., Finger, K., Fricke, C., Gates, M.A., Geiger, H., Geiger-Rudolph, S., Gilmour, D., Glaser, S., Gnugge, L., Habeck, H., Hingst, K., Holley, S., Keenan, J., Kirn, A., Knaut, H., Lashkari, D., Maderspacher, F., Martyn, U., Neuhauss, S., Neumann, C., Nicolson, T., Pelegri, F., Ray, R., Rick, J.M., Roehl, H., Roeser, T., Schauerte, H.E., Schier, A.F., Schönberger, U., Schönthaler, H.-B., Schulte-Merker, S., Seydler, C., Talbot, W.S., Weiler, C., Nüsslein-Volhard, C., and Haffter, P. (1999) A radiation hybrid map of the zebrafish genomeNat. Genet.23(1):86-89.
Nicolson, T., Rüsch, A., Friedrich, R.W., Granato, M., Ruppersberg, J.P., and Nüsslein-Volhard, C. (1998) Genetic analysis of vertebrate sensory hair cell mechanosensation: the zebrafish circler mutantsNeuron20:271-283.