ZFIN ID: ZDB-LAB-001113-2
Nicolson Lab
PI/Director: Nicolson, Teresa
Contact Person: Nicolson, Teresa
Email: nicolson@ohsu.edu
URL: http://www.ohsu.edu/xd/research/centers-institutes/vollum/faculty/faculty-profile.cfm?facultyID=269
Address: Oregon Hearing and Research Center and Vollum Institute Oregon Health & Science University 3181 SW Sam Jackson Park Road Portland, Oregon 97239 USA
Phone: (503) 494-2928
Fax: (503) 494-9612
Line Designation: vo


GENOMIC FEATURES ORIGINATING FROM THIS LAB

STATEMENT OF RESEARCH INTERESTS
Defects in sensory hair cells are thought to account for most forms of hereditary deafness. We aim to identify the components involved in hair-cell function by using a genetic approach. By screening over 7000 genomes (Tuebingen Screens I & II), we have identified a total of 24 genes that are required for balance and hearing in zebrafish. These circler mutants are analogous to the mouse shaker-waltzer mutants and can serve as alternative models for the study of human deafness. We are currently developing new ways of analyzing our mutants such stably expressing hair-cell specific markers, creating behavioral tests for auditory and vestibular function, and measuring physiological responses in hair cells and acousticolateralis nerves.


LAB MEMBERS
Erickson, Tim Post-Doc Maeda, Reo Post-Doc Pacentine, Itallia Graduate Student
Hayashi, Lisa Fish Facility Staff Gibson, Daniel Technical Staff


ZEBRAFISH PUBLICATIONS OF LAB MEMBERS
Sheets, L., Hagen, M.W., Nicolson, T. (2014) Characterization of Ribeye Subunits in Zebrafish Hair Cells Reveals That Exogenous Ribeye B-Domain and CtBP1 Localize to the Basal Ends of Synaptic Ribbons PLoS One9:e107256.
Grisham, R.C., Kindt, K., Finger-Baier, K., Schmid, B., and Nicolson, T. (2013) Mutations in ap1b1 Cause Mistargeting of the Na(+)/K(+)-ATPase Pump in Sensory Hair Cells PLoS One8(4):e60866.
Kalueff, A.V., Gebhardt, M., Stewart, A.M., Cachat, J.M., Brimmer, M., Chawla, J.S., Craddock, C., Kyzar, E.J., Roth, A., Landsman, S., Gaikwad, S., Robinson, K., Baatrup, E., Tierney, K., Shamchuk, A., Norton, W., Miller, N., Nicolson, T., Braubach, O., Gilman, C.P., Pittman, J., Rosemberg, D.B., Gerlai, R., Echevarria, D., Lamb, E., Neuhauss, S.C., Weng, W., and Bally-Cuif, L. (2013) Towards a comprehensive catalog of zebrafish behavior 1.0 and beyond Zebrafish10(1):70-86.
Sheets, L., Kindt, K.S., and Nicolson, T. (2012) Presynaptic CaV1.3 Channels Regulate Synaptic Ribbon Size and Are Required for Synaptic Maintenance in Sensory Hair Cells J. Neurosci.32(48):17273-17286.
Obholzer, N., Swinburne, I.A., Schwab, E., Nechiporuk, A.V., Nicolson, T., and Megason, S.G. (2012) Rapid positional cloning of zebrafish mutations by linkage and homozygosity mapping using whole-genome sequencing Development139(22):4280-4290.
Glover, G., Mueller, K.P., Söllner, C., Neuhauss, S.C., and Nicolson, T. (2012) The Usher gene cadherin 23 is expressed in the zebrafish brain and a subset of retinal amacrine cells Mol. Vis.18:2309-2322.
Einhorn, Z., Trapani, J.G., Liu, Q., and Nicolson, T. (2012) Rabconnectin3alpha promotes stable activity of the h+ pump on synaptic vesicles in hair cells J. Neurosci.32(32):11144-11156.
Kindt, K.S., Finch, G., and Nicolson, T. (2012) Kinocilia mediate mechanosensitivity in developing zebrafish hair cells Dev. Cell23(2):329-341.
Mo, W., and Nicolson, T. (2011) Both pre- and postsynaptic activity of nsf prevents degeneration of hair-cell synapses PLoS One6(11):e27146.
Erickson, T., Pillay, L.M., and Waskiewicz, A.J. (2011) Zebrafish Tshz3b negatively regulates Hox function in the developing hindbrain Genesis49(9):725-42.
Sheets, L., Trapani, J.G., Mo, W., Obholzer, N., and Nicolson, T. (2011) Ribeye is required for presynaptic CaV1.3a channel localization and afferent innervation of sensory hair cells Development138(7):1309-1319.
Trapani, J.G., and Nicolson, T. (2011) Mechanism of spontaneous activity in afferent neurons of the zebrafish lateral-line organ J. Neurosci.31(5):1614-1623.
Trapani, J.G., and Nicolson, T. (2010) Chapter 8 - Physiological recordings from zebrafish lateral-line hair cells and afferent neurons Meth. Cell. Biol.100:219-231.
Mo, W., Chen, F., Nechiporuk, A., and Nicolson, T. (2010) Quantification of vestibular-induced eye movements in zebrafish larvae BMC Neurosci.11:110.
Wright, M.A., Mo, W., Nicolson, T., and Ribera, A.B. (2010) In vivo evidence for transdifferentiation of peripheral neurons Development137(18):3047-3056.
Trapani, J.G., Obholzer, N., Mo, W., Brockerhoff, S.E., and Nicolson, T. (2009) Synaptojanin1 is required for temporal fidelity of synaptic transmission in hair cells PLoS Genet.5(5):e1000480.
Obholzer, N., Wolfson, S., Trapani, J.G., Mo, W., Nechiporuk, A., Busch-Nentwich, E., Seiler, C., Sidi, S., Söllner, C., Duncan, R.N., Boehland, A., and Nicolson, T. (2008) Vesicular glutamate transporter 3 is required for synaptic transmission in zebrafish hair cells J. Neurosci.28(9):2110-2118.
Geisler, R., Rauch, G.J., Geiger-Rudolph, S., Albrecht, A., van Bebber, F., Berger, A., Busch-Nentwich, E., Dahm, R., Dekens, M.P., Dooley, C., Elli, A.F.,Gehring, I., Geiger, H., Geisler, M., Glaser, S., Holley, S., Huber, M., Kerr, A., Kirn, A., Knirsch, M., Konantz, M., Kuchler, A.M., Maderspacher, F., Neuhauss, S.C., Nicolson, T., Ober, E.A., Praeg, E., Ray, R., Rentzsch, B., Rick, J.M., Rief, E., Schauerte, H.E., Schepp, C.P., Schonberger, U., Schonthaler, H.B., Seiler, C., Sidi, S., Söllner, C., Wehner, A., Weiler, C., Nüsslein-Volhard, C. (2007) Large-scale mapping of mutations affecting zebrafish development BMC Genomics8(1):11.
Nicolson, T. (2005) The genetics of hearing and balance in zebrafish Annu. Rev. Genet.39:9-22.
Söllner, C., and Nicolson, T. (2005) The Zebrafish as a Genetic Model to Study Otolith Formation Biomineralization (2nd ed.):229-242.
Seiler, C., Finger-Baier, K.C., Rinner, O., Makhankov, Y.V., Schwarz, H., Neuhauss, S.C., and Nicolson, T. (2005) Duplicated genes with split functions: independent roles of protocadherin15 orthologues in zebrafish hearing and vision Development132(3):615-623.
Söllner, C., Schwarz, H., Geisler, R., and Nicolson, T. (2004) Mutated otopetrin 1 affects the genesis of otoliths and the localization of Starmaker in zebrafish Dev. Genes Evol.214(12):582-590.
Seiler, C., Ben-David, O., Sidi, S., Hendrich, O., Rusch, A., Burnside, B., Avraham, K.B., and Nicolson, T. (2004) Myosin VI is required for structural integrity of the apical surface of sensory hair cells in zebrafish Dev. Biol.272(2):328-338.
Paukert, M., Sidi, S., Russell, C., Siba, M., Wilson, S.W., Nicolson, T., and Grunder, S. (2004) A family of acid-sensing ion channels (ASICs) from the zebrafish: Widespread expression in the central nervous system suggests a conserved role in neuronal communication J. Biol. Chem.279(18):18783-18791.
Söllner, C., Rauch, G-J., Siemens, J., Geisler, R., Schuster, S.C., The Tübingen 2000 Screen Consortium, Müller, U., and Nicolson, T. (2004) Mutations in cadherin 23 affect tip links in zebrafish sensory hair cells Nature428(6986):955-959.
Sidi, S., Busch-Nentwich, E., Friedrich, R., Schoenberger, U., and Nicolson, T. (2004) gemini encodes a zebrafish L-type calcium channel that localizes at sensory hair cell ribbon synapses J. Neurosci.24(17):4213-4223.
Busch-Nentwich, E., Söllner, C., Roehl, H., and Nicolson, T. (2004) The deafness gene dfna5 is crucial for ugdh expression and HA production in the developing ear in zebrafish Development131(4):943-951.
Söllner, C., Burghammer, M., Busch-Nentwich, E., Berger, J., Schwarz, H., Riekel, C., and Nicolson, T. (2003) Control of crystal size and lattice formation by starmaker in otolith biomineralization Science302(5643):282-286.
Concha, M.L., Russell, C., Regan, J.C., Tawk, M., Sidi, S., Gilmour, D.T., Kapsimali, M., Sumoy, L., Goldstone, K., Amaya, E., Kimelman, D., Nicolson, T., Gründer, S., Gomperts, M., Clarke, J.D.W., and Wilson, S.W. (2003) Local tissue interactions across the dorsal midline of the forebrain establish CNS laterality Neuron39(3):423-438.
Sidi, S., Friedrich, R.W., and Nicolson, T. (2003) NompC TRP channel required for vertebrate sensory hair cell mechanotransduction Science301(5629):96-99.
Ernest, S., Rauch, G.J., Haffter, P., Geisler, R., Petit, C., and Nicolson, T. (2000) Mariner is defective in myosin VIIA: a zebrafish model for human hereditary deafness Hum. Mol. Genet.9(14):2189-2196.
Nicolson, T., Ernest, S., Rauch, J., Petit, C., Söllner, C., Seiler, C., and Busch, E. (2000) Isolation of the genes responsible for hearing disorders in zebrafish Eur. J. Neurosci.12:316.
Seiler, C. and Nicolson, T. (1999) Defective calmodulin-dependent rapid apical endocytosis in zebrafish sensory hair cell mutants J. Neurobiol.41(3):424-434.
Geisler, R., Rauch, G.J., Baier, H., van Bebber, F., Brobeta, L., Dekens, M.P., Finger, K., Fricke, C., Gates, M.A., Geiger, H., Geiger-Rudolph, S., Gilmour, D., Glaser, S., Gnugge, L., Habeck, H., Hingst, K., Holley, S., Keenan, J., Kirn, A., Knaut, H., Lashkari, D., Maderspacher, F., Martyn, U., Neuhauss, S., Neumann, C., Nicolson, T., Pelegri, F., Ray, R., Rick, J.M., Roehl, H., Roeser, T., Schauerte, H.E., Schier, A.F., Schönberger, U., Schönthaler, H.-B., Schulte-Merker, S., Seydler, C., Talbot, W.S., Weiler, C., Nüsslein-Volhard, C., and Haffter, P. (1999) A radiation hybrid map of the zebrafish genome Nat. Genet.23(1):86-89.
Nicolson, T., Rüsch, A., Friedrich, R.W., Granato, M., Ruppersberg, J.P., and Nüsslein-Volhard, C. (1998) Genetic analysis of vertebrate sensory hair cell mechanosensation: the zebrafish circler mutants Neuron20:271-283.