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Fig. 3
Competition by steroids and progestins for binding to GQD-GST-zPgrLBD.
Samples were incubated with 2 nM [3H]-17,20β-DHP and 1 μM of competitor. An assay was performed in triplicate for each competitor. The average of the three assays is indicated with standard deviation. Competition for [3H]-17,20β-DHP binding are expressed as a percentage of maximum specific progesterone (P4) binding. The competitors analyzed were progesterone (P4); 17,20β-DHP; Org OD 02; 17β-estradiol (E2); testosterone (T); Cortisol.
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