Fig. 1

Fig. 1

Expression and purification of the ligand-binding domain (LBD) of zebrafish Pgr as a fusion protein with GST and production of GQD-coupled GST-zPgrLBD.

A. A diagram of the produced recombinant GST-fusion zPgrLBD. The predicted ligand-binding domain at the C-terminus is indicated by black boxes. The GST tag at the N-terminus is indicated by a white box. B. SDS-PAGE analysis of purified recombinant GST-zPgrLBD. Protein bands were detected by CBBR staining (CBBR) or immunostained with anti-GST-tag antibody (α-GST). C. Western blot analysis of GST-zPgrLBD and GQD-GST-zPgrLBD. The protein band of GST-zPgrLBD is indicated by an arrowhead. The bands of GQD-GST-zPgrLBD are indicated by a parenthesis.