IMAGE

Fig. 2

ID
ZDB-IMAGE-240402-8
Source
Figures for Liang et al., 2024
Image
Figure Caption

Fig. 2

Csrp3 is involved in trabeculation, and its expression is regulated by hemodynamics and Notch signaling during heart development. A Upper: visualization of trabeculae using Tg(vmhc:mCherry-NTR) displayed increased trabeculation in 218A larval hearts. Scale bars, 20 μm. Bottom: Quantification of the number or density of trabeculae in wild-type and 218A larval hearts at indicated stages. N = 9 each. Data are presented as mean ± SD, Student’s t-test, **, p < 0.01. B Upper: HE staining showed an increased myocardial density in 218A adult hearts. Scale bars, 100 μm. Bottom: Quantification of the myocardial density in wild-type and 218A adult hearts. N = 6 each. Data are presented as mean ± SD, Student’s t-test, ***, p < 0.001. C Comparison of the fluorescence pattern in the hearts of + /218A and Tg(tp1:d2GFP) larvae. Scale bars, 20 μm. D, E WISH and confocal images showed that inhibition of Notch signaling with DATP treatment enhanced both endogenous csrp3 expression in wild-type hearts (D) and GFP fluorescence in + /218A hearts (E). Scale bars, 20 μm. F Confocal and WISH images showed that reducing blood flow via tnnt2a MO injection markedly suppresses both GFP fluorescence in + /218A hearts and endogenous csrp3 expression in wild-type hearts. Scale bars, 20 μm

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell. Mol. Life Sci.