Fig. 1

Fig. 1

gSAIzGFFM218A directs GFP expression in zebrafish hearts and harbors an insertion in the csrp3 genomic locus. A Mosaic GFP expression in the hearts of heterozygous gSAIzGFFM218A (+ /218A) larvae and juveniles at indicated stages. Scale bars, 50 μm. B GFP in + /218A overlapped with the myocardial marker (cmlc2:mCherry reporter), but neither with the endocardial marker (flk:mCherry reporter) nor the epicardial marker (anti-Pck staining). Scale bars, 20 μm. C Schematic diagram illustrated the components of the gene-trap construct and its insertion in intron 3 of the csrp3 locus, which was predicted to result in a truncated csrp3 transcription. D Sequencing analysis of csrp3 cDNA from wild-type or homozygous 218A larvae. E RT-PCR analysis of csrp3 transcripts from wild-type, + /218A, and 218A larvae using specific primers indicated in C. M, molecular weight markers. F Western blotting revealed a dramatic reduction in Csrp3 protein level in 218A mutants. G Whole-mount in situ hybridization (WISH) showed the endogenous csrp3 expression in wild-type or 218A larval hearts. Scale bars, 50 μm