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Fig. 1 Maternal expression of adamts9 in zebrafish embryos. A–D, Maternally-deposited EGFP signal in progeny from crosses of female Tg(adamts9:EGFP)ecu19 transgenics with wild-type males (WT, AB strain). Merged confocal images (EGFP and T-PMT channels) of embryos at the 1-cell, 2-cell, 4-cell, and 10 hours post-fertilization (hpf) stages are shown. Similar expression was confirmed in multiple F3 embryos from five independent F2 transgenic lines. E,G, No observable EGFP signal prior to 10 hpf in early dividing embryos from crosses of male Tg(adamts9:EGFP)ecu19 transgenics with WT females. H, zygote adamts9:EGFP expression. Scale bar: 200 μm.