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Fig. 8 adamts9 signal in adult gonad of zebrafish. A–C, adamts9:EGFP expression. Representative merged confocal images (EGFP and T-PMT channels) of ovaries from adult female zebrafish. No observable EGFP signal in fully-grown but immature follicles (stage IVa, A). Strong EGFP signals were found in preovulatory follicles (stage IVb, B, and C). dpf, days post fertilization. D–I, immunohistochemistry staining with an anti-Adamts9 primary antibody in adult ovaries (D–F) or adult testes (H,I). G, Negative control staining without the primary antibody. Scale bar: 200 μm (A,B); 50 μm (E,G–I); 10 μm (C,D,F).