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Fig. 1 TMEM47 is stimulated by virus infection. (A–D) Quantitative PCR (qPCR) detection of the transcriptional levels of tmem47, ifnφ1, and irf7 on SVCV or CyHV-2 stimulation. ZF4 cells (A and B) or ZFL cells (C and D) were seeded on six-well plates overnight and stimulated with SVCV [multiplicity of infection (MOI) = 1] or CyHV-2 (MOI = 0.01). At the time points 0, 6, 12, 24, and 48 h, total RNAs were extracted for further qPCR assays. β-actin was used as an internal control for normalization, and the relative expression is represented as fold induction relative to the expression level in control cells (set to 1). Data were expressed as mean ± standard error of the mean (SEM), n= 3. Asterisks indicate significant differences from control (*P < 0.05). All experiments were repeated for at least three times with similar results.