Fig. 1

Fig. 1 Effects of stress on phenotypic variability are captured via individual animal hashing of single-cell transcriptomes (A) Representative images of 24 hpf embryos raised at standard and elevated temperature; individual embryos with normal-looking and bent-tail phenotypes were included in the dataset. (B) Experimental workflow for temperature perturbation experiment and individual embryo hashing. (C) Uniform manifold approximation and projection (UMAP) of temperature-perturbation dataset, projected into coordinate space of reference atlas (see Saunders et al.32). Right side shows how single-cell data are transformed to generate cell composition matrices.