|
Fig. 2 gata2a is required for lineage specification and transcriptional regulation in WKM hematopoietic populations (A) scRNA-seq (left) and scATAC-seq (right) t-distributed stochastic neighbor embedding (tSNE) clustering of WKM hematopoietic populations. Analysis estimated 3,047 WT cells and 5,584 gata2a−/− cells for scRNA-seq (from 3 WT/gata2a−/− fish pooled to give one WT/mutant [MUT] sample that was sequenced) and 10,004 WT cells and 6,500 gata2a−/− cells for scATAC-seq (n = 3/condition [biological replicates]). Multi, multiciliated kidney (stromal) cells; cHSPCs, core HSPCs; Neut, neutrophils; NK/T, natural killer and T cells; Mono/Macs, monocytes/macrophages; Ery, erythroytes; mHSPCs, myeloid-biased HSPCs; Ery-P, erythroid progenitors; Endo, endothelial cells. Full list of differentially expressed genes can be found in Table S2. (B) Percentage of WKM populations of total WT or gata2a−/− cells. (C) Chromatin accessibility near promoter regions (scATAC-seq) and RNA expression (scRNA-seq, RNA assay) of indicated genes. Each dot represents a cell. cHSPC, core HSPC; mHSPC, myeloid-biased HSPC; Ery-P, erythroid progenitors. See also Figures S1 and S2 and Tables S1 and S5.