Figure 2.

Figure 2. Characterization of functional response types during habituation learning.

(A) Heatmap of the response profiles of ROIs categorized into 12 functional clusters. n = 16,607 ROIs from 34 larvae. (B) Average z-scored fluorescence of each functional cluster plotted for the whole experiment (left column) and centred on each dark flash (DF) stimulus (right column), demonstrating the differences in both Adaptation Profiles and Response Shape for each cluster. Clusters were identified using Affinity Propagation clustering (affinity = Pearson correlation, damping = 0.9, preference = -9), and organized using hierarchical clustering, distance = complete, correlation. Dashed lines in the top panels are the DF stimulus convolved with a kernel approximating H2B-GCaMP7f kinetics, used as the regressor in the analysis. (C) Summed intensity projection of the ROIs belonging to each functional cluster in Z-Brain coordinate space depicting their physical locations in the brain. Projection images are normalized to the maximum value. (D) Heatmap depicting the density of each cluster that is found within different Z-Brain regions. (E) Correlogram calculated from the Pearson correlation in downsampled volumes for the ROI centroid positions for each cluster (see ‘Methods’). Hierarchical clustering, distance = complete, correlation. (F) Correlation between motor events and the Ca²⁺ traces for each ROI assigned to the functional clusters. dots = individual ROIs, bar height = 99.99999% confidence interval around the median value.