Figure 4

Figure 4

The locomotor activity of brsk2ab^−/− zebrafish in different stages. (A) The trajectory of locomotion in WT and brsk2ab^−/− zebrafish in different time points. (B) The brsk2ab^−/− zebrafish presented significantly reduced swimming velocity at 5 dpf (WT vs. brsk2ab^−/−, 3.342 ± 1.63 vs. 2.229 ± 1.18; p = 0.0002). While no significant difference was observed at 14 dpf (4.046 ± 1.50 vs. 3.826 ± 1.18; p = 0.2753) and later stages (2 mpf: 5.589 ± 1.47 vs. 6.234 ± 1.75; p = 0.1087; 3 mpf: 6.309 ± 1.31 vs. 5.998 ± 1.37; p = 0.2745). At 5 dpf: n = 48: 48; at 14 dpf: n = 89: 91; at 2 mpf: n = 26: 20; and at 3 mpf: n = 25: 25. Data are shown as mean ± SEM, *** p < 0.0003.