ZFIN Image: Finotto et al., 2023, Figure EV5

Image

Figure Caption

Figure EV5 LGALS1 is involved in suppression of the immune system

A

Violin plot showing CLU expression levels in GSCCs.

B

Violin plot showing TREM2 expression levels in macrophages (P = 0.8611 (LBT003), 0.5895 (LBT070), 0.3979 (BT333), 0.6995 (LBT001), 0.0122 (CME037), 0.0526 (BT569), 0.0028 (LBT123), 2.8e‐8 (CME038)).

C

Representative immunofluorescence images of LBT070 LGALS1 WT and LBT070 LGALS1 KO cells showing expression of GAL1 (green), and cell nuclei stained by DAPI. Scale bars: 100 μm.

D, E

Maximum intensity projections of a z stack of the head region of Tg(mpeg1:mCherryF)^ump2; Tg(kdrl:lynEYFP)^md77 zebrafish embryos with GFP‐labeled LBT070 (D) and LBT070 LGALS1 KO (E) tumors at 5 dpi. GBM tumor cells are shown in green, GAMs in red, and blood vessels in blue. Scale bars: 50 μm. n = 9 (D) and 5 (E).

F

Boxplot of GAM distance to the tumor of all GAMs within 30 μm of the tumor, at the start of 1 and 5 dpi time‐lapse movies (n = 257 and 99 (LBT070; 1 and 5 dpi), 481 and 83 (LBT070 LGALS1 KO; 1 and 5 dpi); P = 0.0020 (LBT070), 0.9997 (LBT070 LGALS1 KO), 0.8013 (1 dpi), 0.0067 (5 dpi); boxes stand for 50% of the data and minima/maxima are indicated by the line ends).

Data information: The zebrafish experiments describe biological replicates, i.e. individual zebrafish embryos. The xenograft experiment was replicated twice for LBT070 LGALS1 KO, and three times for LBT070. In (B), the P‐values were calculated by Kruskal–Wallis test (P = 2.5e‐10). In (F), the P‐values were calculated by two‐way ANOVA, followed by pairwise testing with Tukey's multiple comparisons correction. ns ≥ 0.05, *P < 0.05, **P < 0.01, ****P < 0.0001.

Source data are available online for this figure.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ EMBO Mol. Med.