|
Fig. 2 Uptake of different sized AuNPs into the pronephros of zebrafish measured by fluorescence detection with SPIM. Uptake was measured in both sides of the zebrafish pronephros (white markers – right side, black markers – left side). Fish were imaged using SPIM at 4 dpf after 72 h exposure to AuNPs of sizes (a) 10 nm, (b) 20 nm, (c) 40 nm, (d) 80 nm, and (e) 100 nm. Raw data are represented as a scatter plot with means shown (indicated by the red line) for three independent experiments n = 18. (****P < 0.0001, **P < 0.01, *P < 0.05 and ns = not significant). Magnified versions of the graph are provided as figure insets for the 10 nm, 20 nm and 100 nm AuNPs to best visualise the comparatively low levels of particle uptake/fluorescence for these exposures.