ZFIN Image: Kolvenbach et al., 2022, Fig. 4

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Figure Caption

Fig. 4 Phalloidin stainings and SR101 excretion assay show cloacal obstruction in shroom4 morphants. (A,B) Cloacal region is depicted at 5 dpf. White arrows indicate the hindgut; the white arrowheads show the distal pronephric ducts. With shroom4 MO-injected zfl display a dilated hindgut and distal pronephric morphology compared with Ctrls. (C,D) KD with shroom4 MO causes an abnormal cloacal excretion of SR101 compared with Ctrl MO-injected zfl at 5 dpf elucidated by white arrowheads (C,D). (E–G) shroom4 morphants show significantly longer time to excretion (E), significantly reduced number of excretions over time (F), and oscillating or missing peristalsis compared with Ctrls (G) (n=3). Scale bars represent 30 µm (A,B) and 100 µm (C,D). Ctrl, control; MO, morpholino oligonucleotide; SR101, sulforhodamine 101; zfl, zebrafish larvae.

Acknowledgments

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