Fig. 2.

Fig. 2.

Melanophores require kcnj13 autonomously during stripe formation. (A-C) Testing cell-autonomy of kcnj13 by blastula transplantations reveals a genetic requirement in melanophores (A; kcnj13^td15;ednrba^tlf802;csf1ra^tm236b into mitfa^w2; n=1), but not in xanthophores (B; kcnj13^td15;kita^b134;ednrba^tlf802 into csf1ra^tm236b; n=2) or iridophores (C; kcnj13^td15;mitfa^w2;csf1ra^tm236b into ednrba^tlf802; n=1). (D-D″) Transplantation experiments (kcnj13^tui24 into slc45a2^b4) provide further evidence of a cell-autonomous function of kcnj13 in melanophores during stripe formation. Transplanted mutant melanophores (pigmented) are associated with stripe perturbations in slc45a2 hosts (n=3). Strong pattern deformations are never observed in chimeras without pigmented trunk melanophores (n=41). Control transplantations of wild-type melanophores into slc45a2^b4 hosts do not cause such pattern deformations (Dooley et al., 2013a,b).