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Fig. 6 Biodistribution of lipoplexes composed of fluorescently labeled liposomes (megenta, DOTAP/DOPE/DSPE-Rhodamine /C14-pSar2k at a molar ratio of 48.65/48.65/0.2/2.5, 30 mM) and Atto488 labeled luciferase mRNA (green, 150 pg mRNA, N/P 10) (A) in brain after intraventricular injection and (B) in whole embryo after intravenous injection in 2.5 dpf ABTL zebrafish embryos. Confocal images were taken by 10× lens for whole embryos view (scale bar: 100 μm) and 40× lens (scale bar: 20 μm) at 1 hpi, 2 hpi, 4 hpi and 24 hpi. Co-localized liposomes and mRNA were shown in red square. Free liposomes were marked by orange arrows in the zoomed images, respectively. F: forebrain; M: midbrain; H: hindbrain. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)