Image
|
Figure Caption
Fig. 4
Validation of lgals3bpb as the 4C4 antigen by recombinant protein expression in HEK293T cells. (A-E) Immunofluorescence of HEK293T cells (A) or following transfection with the empty pcDNA3 plasmid (B), lgals3bpb (C,D), zgc:112492 (E) or ependymin (F) coding sequences. Bright field (PMT), 4C4 staining (green) and 4C4 with DAPI staining (blue) staining channels are shown (three independent experiments). (D) High magnification from pcDNA3-lgals3bpb-transfected cells. Scale bar 10 μm. Image was taken using a 40× water-immersion objective and a numerical zoom of 3. A c-myc tag (magenta) was used to validate the endogenous expression of the transfected genes (two independent experiments). Scale bars 100 μm. Images were taken using a 20× objective
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Full text @ Dev. Dyn.