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Fig. 2
Multiplexed HCR in situ labeling of single cells.
Example of a single focal plane of a multiplexed HCR labeled fish. The endogenous expression of Tg(elavl3:H2b-GCaMP6s) labels neuronal nuclei and can be used to segment single neurons and to identify whether genes (here cart2 and cart3) are coexpressed (top right panel, arrowheads) or not (bottom right panel). Scale bars, 100 μm (left) and 10 μm (right).
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