ZFIN Image: Everson et al., 2022, Fig. 4

Image

Figure Caption

Fig. 4

PBO and alcohol interact to synergistically reduce fli1:EGFP expression. (a–d) Fluorescent images of 26 h post fertilization (hpf) fli1:EGFP embryos exposed to control or the indicated concentration of PBO, ethanol, or both from 6–24 hpf showing a synergistic interaction between ethanol and PBO. (e) Column graph showing fli1:EGFP fluorescence measurements for each embryo (open circles). Black bars show mean fluorescence for each group ± SEM. Sample sizes: control n = 17; 1% ETOH n = 17; 25 μM PBO n = 14; ETOH+PBO n = 17. Statistic: two‐tailed ANOVA with Tukey's multiple comparison's correction between all groups. **** p < .0001. Scale bar = 0.2 mm. RFU = relative fluorescence units. ETOH = ethanol. PBO = piperonyl butoxide, environmental Hedgehog pathway inhibitor

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Birth Defects Res