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Figure 1—figure supplement 1. <italic toggle='yes'>Control experiments for speck formation in muscle cells with Opto-ASC and with mutated Cry2olig in periderm cells</italic>.

(A) Opto-ASC Tg(mCherry-Cry2olig-asc) specks induced by blue light (488 nm) in muscle cells in 3dpf larva. Multiple oligomers are formed by the end of the movie (60 mins). Opto-ASC is in black. Scale bar is 50 μm. (B) Periderm cells in a 3dpf larva expressing mCherry-Cry2oligR498-ASC (in red). The mutated version of Cry2olig does not form oligomers even after 50 min of constant illumination by blue light (488 nm). Endogenous ASC-GFP in green. Scale bar is 50 μm.

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