ZFIN Image: Zhang et al., 2023, Fig. 1.

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Figure Caption

Fig. 1.

PCR tagging in zebrafish. (A) PCR tagging workflow: a gRNA/Cas9 RNP targeting the start or stop codon of a gene of interest is co-injected with a PCR-generated HDR template with short homology arms into fertilized zebrafish eggs, leading to integration of a fluorescent protein (FP) coding sequence. UTR, untranslated region. Red box indicates field of view in B,C and throughout the figures. (B,C) Lateral view of spinal cords of 3-5 dpf vamp2-mCherry and mEGFP-vamp1 PCR-tagged zebrafish showing vesicular fluorescence in synaptic boutons (arrowheads). Scale bars: 5 µm.

Acknowledgments

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