Fig. 3

Fig. 3 Stat3 is necessary for Hif1α transcriptional activity.

A Representative pictures of HRE:mCherry reporter zebrafish treated with AG490 in combination with low oxygen tension, DMOG, and Dex from 3-6 dpf and CoCl₂ from 5-6 dpf. Scale bar 500 μm. n = 18 DMSO normoxia, 11 DMSO hypoxia, 18 AG490, 9 AG490 hypoxia, 11 Dex, 11 Dex AG490, 12 DMOG, 12 DMOG AG490, 18 CoCl₂, 12 CoCl₂ AG490 (larvae used for this experiment came from three independent breeding between wild type zebrafish). B Fluorescence quantification of HRE:mCherry reporter zebrafish treated with AG490 in combination with low oxygen tension. C Fluorescence quantification of HRE:mCherry reporter zebrafish treated with AG490 in combination with 10 μM Dex. D Fluorescence quantification of HRE:mCherry reporter zebrafish treated with AG490 in combination with 50 μM DMOG from 3–6 dpf. E Fluorescence quantification of HRE:mCherry reporter zebrafish treated with AG490 in combination with 0.1 mM CoCl₂ from 5-6 dpf. F, G Representative pictures and fluorescence quantification of HRE-mCHerry reporter zebrafish larvae treated with 12.5 μM PD98059 in combination with low oxygen tension. n = 31 DMSO normoxia, 29 PD98059 normoxia, 28 DMSO hypoxia, 29 PD98059 hypoxia (larvae used for this experiment came from three independent breeding between wild type zebrafish). Scale bar = 500 μm. H, I Representative pictures and fluorescence quantification of stat3^+/+, stat3^+/− and stat3^−/− sibling larvae in HRE:mCherry transgenic background treated with low oxygen tension from 3–6 dpf. n = 7 stat3^+/+ normoxia, 9 stat3^+/+ hypoxia, 6 stat3^+/− normoxia, 7 stat3^+/− hypoxia, 8 stat3^−/− normoxia, 6 stat3^−/− hypoxia (larvae used for this experiment came from three independent breeding between stat3^+/− zebrafish). Scale bar = 500 μm. Mean ± SEM.