Fig. 3

Fig. 3 Medaka/zebrafish Bncr chimeras reveal specificity determinants in fingers 2 and 3.

A Zebrafish (blue) and medaka (dark yellow) mature Bncr protein sequence alignment and schematic of the Bncr protein fold. Fingers are labeled 1, 2, and 3 and correspond to the amino acids in boxes in the protein sequence alignment. Amino acid sites are numbered starting with the first site in the mature protein. Note that each finger is bounded by cysteine residues that keep disulfide bridges intact. “Top” and “base” are indicated. B Comparative medaka/zebrafish IVF data with Bncr chimera lines. N-glycosylation pattern of each chimera is depicted with sugar chain symbols. Means ± SD are indicated. (Two-tailed Wilcoxon matched-pairs signed rank test with the method of Pratt; ns not significant). C Plot of the bias index values derived from the IVF data in (B). Bias could not be calculated for data pairs for which the fertilization rates with both sperm were equal to 0. Means ± SD are indicated. (Two-tailed Wilcoxon signed rank test vs. theoretical median of 0 with the method of Pratt).