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Fig. 4
Effects of penisterine D (4) on tube formation and cytotoxicity of human EPCs. Cells were treated with the indicated concentrations of penisterine D and sorafenib for 24 h. (A) The inverted phase-contrast microscope was utilized for recording tubular morphogenesis. (B) The tube formation of EPCs was quantified by measuring the length of tubes by Image-J. (C) Cells were treated with 4 for 24 h and cytotoxicity was determined using the LDH assay. Data represent the mean ± SEM. * p < 0.05, compared with the control group.
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