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Figure S4.
(A) Shown are the schematic representations of lipophilic fluorescent dye uptake in the head (amphid) and tail (phasmid) sensory neurons in C. elegans. Red labelling indicates the normal dye uptake, whereas the failure of dye uptake was shown in white. Fluorescence images show the dye update in the head and tail neurons in the WT and indicated mutant strains. No dye uptake was observed in both head (amphid) and tail (phasmid) of wdr-31(tm10423);elmd-1 double mutants, wdr-31(tm10423);elmd-1;rpi-2 and wdr-31(syb1568);elmd-1;rpi-2 triple mutants. Ex[elmd-1(+)] rescues the dye uptake defects of wdr-31(tm10423);elmd-1 double and wdr-31(tm10423);elmd-1;rpi-2 triple mutants, whereas Ex[wdr-31(+)] rescues the dye uptake defects of wdr-31(syb1568);elmd-1;rpi-2 triple mutants. Scale bars: 10 μm. (B) Shown is the jitter plot for AWB long cilia length (μm) in WT and mutant strains.