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Figure 5
(A) Time required for peak (or max) sheath accumulation per cell (hours). Error bars are standard deviation. (B–F) Videos of n=19 dorsal cells (19 larvae) and n=18 ventral cells (18 larvae) were quantified for sheath initiation and loss in each 5 min frame. The time-lapse videos were then normalized for quantitative comparison by defining the video frame at which each cell accumulated its peak (or max) number of immature ensheathments as T0’. Each frame before or after that was –5 min or +5 min. The vertical dashed line aligns the T0’ time point of each graph in B, D, and F. (B) The average number of immature ensheathments for dorsal and ventral cells that are present in each frame is plotted based on time relative to T0’; error bars represent SEM. (C) Percent of ensheathment attempts from the entire imaging period that occurred prior to reaching peak. Error bars represent SEM. (D) The average number of new ensheathment attempts in each frame is plotted with the same time normalization as in B. Error bars represent SEM. (E) Similar to C, the percent of ensheathments from the entire imaging period that were lost prior to reaching peak. Error bars represent SEM. (F) The average number of ensheathments that were lost in each frame is plotted with the same time normalization as in B. Error bars represent SEM. Individual data points are shown and significance was determined by Mann-Whitney tests in A, C, and E. The data in B, D, and F was cropped at +6.5 hr (see associated source data).
Oligodendrocyte nascent sheath accumulation involved a consistent and high rate of sheath initiation and loss.