Figure 2
ISH at 11 hpf after bmp4-induction at 8.5 hpf. left columns wildtypes, right columns bmp4-induced. first and third column dorsal view, second and fourth column lateral view. (A): summary of experimental procedure. Embryos were heat-shocked at 8.5 hpf and analyzed at 11 hpf. (B–E): Expression of emx3 is condensed at the midline after bmp4-induction (arrows). (F–I): the foxg1a domain in the forebrain (arrow) is lost after bmp4-induction. (K–N): expression of eye-field marker six3b is also condensed at the midline after bmp4-induction. (O–R): expression of eye-field marker rx3 (arrow) is lost after bmp4-induction. Scalebars indicate 200 µm.