ZFIN Image: Okeke et al., 2022, Fig. 2

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Figure Caption

Fig. 2 Behavior of non-ectomesenchyme and early NC genes in nr2f2^el/el;nr2f5^el/el mutants. (A) In situ hybridizations analysis showing that markers of alternate lineages are not overtly upregulated at 20 hpf. (B) In situ hybridizations for early NC genes in control and double mutants at 20 hpf. Arrowheads indicate elevated sox10 expression. In A,B, top right numbers are the average TPM values in the RNAseq data, with the log2-transformed fold-change DESeq2 values underneath. (C) Immunostaining showing ectopic Sox10 protein in the arches (outlined) at 25 hpf. sox10:kikGR marks NC-derived cells. Scale bars: 100 µm.

Acknowledgments

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