ZFIN Image: Pose-Méndez et al., 2023, Figure 6

Image

Figure Caption

Figure 6

Monitoring of the Purkinje cell (PC) layer after induced PC ablation in adults.

(A) In vivo stereomicroscopy showing tagRFP-T fluorescence in the PC layer, and illustration of the time course of fluorescence recovery after PC ablation in adults (at 5 mpf) monitored until 1 ypt. Representative confocal images of whole mount cerebelli from dorsal view (B–E) and sagittal vibratome sections after immunostaining with the antibodies anti-tagRFP and anti-ZebrinII (F–K), comparing the cerebellum in ablated fish (endoxifen treated; C–E, F–H) vs control group (dimethyl sulfoxide [DMSO] treated; B, I–K). Arrows in C, F point out apoptotic bodies. Equivalent results were observed in two additional independent ablations in adult cerebelli (data not shown).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife