ZFIN Image: Shih et al., 2023, Figure 4

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Figure Caption

Figure 4

Effects of low-Na⁺ acclimation on the number of NCC ionocytes and Ncc2 expression within individual NCC ionocytes in the gills of adult zebrafish. The cell number of NCC ionocytes and the fluorescence intensity of Ncc2 signals in the gill filaments were analyzed by whole-mount IF after acclimation to low-Na⁺ FW (a). NCC ionocyte number of filaments (N = 12) (b), mean fluorescence intensity of individual NCC ionocytes (N = 531–1017) (c), and the average mean fluorescence intensity of NCC ionocytes of filaments (N = 12) (d) are shown. Values are the mean ± SD or SEM. Student’s t-test or Mann–Whitney U test, *** p < 0.001, **** p < 0.0001. Scale bar, 50 μm.

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