Fig. 4

Fig. 4 Fig. 4. Comparison of behavior endpoints between untreated control, arecoline, arecaidine, guvacine, and guvacoline exposed zebrafish with mirror biting and predator avoidance tests. (A) Mirror biting time percentage and (B) longest duration in the mirror side were analyzed during the mirror biting test while (C) approaching predator time and (D) average distance to predator’s separator were analyzed during the predator avoidance test. The data are expressed as the median with interquartile range and were analyzed by Kruskal-Wallis test continued with uncorrected Dunn’s test (n = 30 for the control, Guvacine, and Guvacoline treatment groups; n = 29 for Arecaidine treatment groups, n = 27 for Arecoline treatment groups, * P < 0.05, ** P < 0.01, *** P < 0.001).