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Figure 6

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ZDB-IMAGE-230228-180
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Figures for Sato et al., 2023
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Figure Caption

Figure 6

Effect of acetaminophen on hepatocyte differentiation in rat CYP2E1-TG larvae. EGFP fluorescence-negative (EGFP [−]) (A,C) and -positive (EGFP [+]) (B,D) F2 CYP2E1-transgenic larvae were exposed to 2.5 mM acetaminophen (APAP) from 48 hpf to 54 hpf. The larvae were fixed at 54 hpf for whole-mount in situ hybridization with a foxa3 probe, a marker for the liver primordium. All images (AD) are of a dorsal view, and black arrows indicate the expression area of foxa3. Scale bars: 200 µm. Panel (E) indicates averaged areas of foxa3 expression (n = 5–9). The “a” over each bar indicates that these values are not statistically different.

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