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Fig. 3
Fig. 3. In situ hybridization confirms erbb3b expression in GFP+ cells. (A) Whole-mount, in situ hybridization for erbb3b in gSAIzGFFD37A fish at 24 hpf. Anterior and posterior lateral line ganglia indicated by stars and trunk neural crest cells by arrowheads. Sections of (B) erbb3b and (C) sox10 in situ hybridization after antibody labeling of GFP (arrows indicate areas of colocalization between the respective mRNA transcript and erbb3b-driven fluorophore expression). Scale bars, 20 μm unless otherwise noted.
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Reprinted from Developmental Biology, 482, Brown, E.A., Kawakami, K., Kucenas, S., A novel gene trap line for visualization and manipulation of erbb3b+ neural crest and glial cells in zebrafish, 114-123, Copyright (2021) with permission from Elsevier. Full text @ Dev. Biol.