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Fig. 2
One-pot 3’-end RNA labelling. (A) Reaction scheme. Conditions: i) pU3 (140 µM), aqueous NaIO4 (1.4 mM), 30 min, and 25°C in the dark. ii) Addition of R-EDA (1.0 mM) and NaBH3CN (20 mM) in KH2PO4 buffer (0.1 M pH 6.0). HPLC-determined yields after two hours of reductive amination are presented for each N-nucleophile. (B) HPLC chromatograms of reaction with Cy3-EDA at different stages. (C) Second-order reaction rate constant k values determined for reductive amination reaction between pU3-dial (100 µM) and different N-nucleophiles (1 mM).
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Full text @ Nucleic Acids Res.