ZFIN Image: Bertozzi et al., 2021, Fig. 6

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Figure Caption

Fig. 6

Fig. 6. Inhibition of Wnt/β-catenin signaling prevents cardiomyocyte dedifferentation. (A) embMHC and MF20 immunostaining on cryoinjured hearts of hs:Axin1-YFP fish and wild-type siblings, heat-shocked daily from 1 to 7 dpi. n (hearts) = 7 (wild-type), 8 (hs:Axin1-YFP). In the magnified view, the cyan dotted lines represent the outline of the MF20⁺ cardiomyocyte area. Scale bars, 100 μm (overview), 10 μm (magnification). (B) Quantification of the embMHC⁺MF20⁺/MF20⁺ area in the 50 μm wound borderzone of images shown in (A). Error bars represent CI 95%. Unpaired Welch's unequal variances t-test. (C) MF20 and GFP immunostaining on cryoinjured hearts of −14.8gata4:GFP fish treated with DMSO or IWR1 from 1 to 7 dpi. n = 8 (DMSO), 6 (IWR1). Scale bars, 100 μm (overview), 10 μm (magnification). (D) Quantification of GFP⁺MF20⁺/MF20⁺ area in the 50 μm wound borderzone of images shown in (C). Error bars represent CI 95%. Unpaired Welch's unequal variances t-test.

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Reprinted from Developmental Biology, 481, Bertozzi, A., Wu, C.C., Hans, S., Brand, M., Weidinger, G., Wnt/β-catenin signaling acts cell-autonomously to promote cardiomyocyte regeneration in the zebrafish heart, 226-237, Copyright (2021) with permission from Elsevier. Full text @ Dev. Biol.