Fig. 1

Fig. 1

Fig. 1. Wnt/β-catenin signaling is active in cardiomyocytes at the wound border in the cryoinjured zebrafish heart. (A) axin2 and lef1 expression levels in the whole ventricle of sham-injured and cryoinjured heart at 7 days post cryoinjury (dpi), measured by RT-qPCR. Values are plotted as ΔΔCt relative to sham-injury. Error bars represent s.e.m. n (biological replicates) ​= ​3 (sham injury), 3 (7 dpi). 3 hearts were pooled for each sample. Numbers indicate p-value determined by unpaired Welch's unequal variances t-test. (B) GFP immunofluorescence in cardiac troponin T (cTnT)⁺ cardiomyocytes from the wound borderzone or the remote area of the myocardium in sham-injured and 7 dpi hearts of 7xTCF-Xla.Sia:GFP^ia4Tg fish. n (hearts) ​= ​7 (sham injury), 7 (7 dpi). In the overview, the cyan and yellow dashed squares represent the magnified portion of the myocardium from the remote area and the borderzone, respectively. In the magnified view, the cyan and yellow dotted lines represent the outline of the cTnT⁺ cardiomyocyte area. Scale bars, 100 ​μm (overview), 10 ​μm (magnification). (C) axin2 RNAscope signals in MF20⁺ cardiomyocytes from the borderzone or the remote area of the myocardium of sham-injured and 7 dpi hearts. Scale bars, 100 ​μm (overview), 10 ​μm (magnification). (D) Quantification of axin2 RNAscope signals in borderzone cardiomyocytes of hearts after sham injury and at 3, 7, 15 and 30 dpi. Error bars represent CI 95%. n (hearts) ​= ​6 (sham-injury), 6 (3 dpi), 5 (7 dpi), 4 (15 dpi), 4 (30 dpi). One-way ANOVA with Dunnett's multiple comparisons test.