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Fig. 6
m-optoSynC activation in murine hippocampal neurons blocks synaptic transmission.
a Plot showing changes in normalized fluorescence intensity of mOrange2 by electrical field stimulation of neurons expressing mOrange2-SYP before (black line) and after 488 nm illumination for 30 s (green line). N = 2 independent cultures. n = 5 neurons and 116 synapses. b, c Normalized fluorescence intensity at 2 s or 25 s after electrical stimulation. Paired t-test. d–f Same as a-c, respectively, but in neurons expressing m-optoSynC (CRY2olig(535) inserted into SYP-mOrange2. Paired t-test. N = 3 independent cultures. n = 10 neurons, and 108 synapses. ns = not significant. *<0.05.
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