Fig. 9

Dexamethasone exposure increased hair cell sensitivity to neomycin.A–J Representative maximum intensity images of fixed pLL neuromasts with DAPI-labeled hair cell nuclei (blue). Larvae (7 dpf) were exposed for 48 h to DMSO (carrier) alone (A–E) or dexamethasone (F–J), then exposed for 30 min or 10 µM (B, G), 30 µM (C, H), 50 µM (D, I), or 100 µM (E, J) neomycin. EM buffer alone was used as a control (A, F). K Dose–response curve with mean and SEM bars shows the number of hair cells per neuromast as a percent of those present in unlesioned controls. Dexamethasone-treated neuromast hair cells were more sensitive to neomycin-induced cell death than controls (adjusted *P < 0.05 (50 µM), ****P < 0.0001 (100 µM)). N = 7 fish per condition per trial; 3 experimental trials