ZFIN Image: Nie et al., 2022, Figure 5.

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Figure 5.

Comparative transcriptome analysis of runx2b^+/+ and runx2b^−/− zebrafish, and overexpression and knockdown analysis of runx2b. (A) In situ hybridization of runx2b in the tissue of origin of IBs (IBs, shown with arrowheads). The expression of runx2b was significantly reduced in runx2b^−/− zebrafish. (B) UMAP visualization of tendon progenitors, mature tenocytes, differentiating tenocytes and osteoblasts in runx2b^+/+ and runx2b^−/−zebrafish. The ratios of osteoblasts to the total number of cells in runx2b^+/+ and runx2b^−/− zebrafish were 2.44% (340 cells) and 0.31% (34 cells), respectively. (C) Violin plots showing expression of differentially expressed genes in each cluster related to IB formation between runx2b^+/+ and runx2b^−/−zebrafish. (D, E) Overexpression and knockdown analysis of runx2b in IB cells of M. amblycephala. The expression levels of genes related to osteoblasts were detected by qRT-PCR. ANOVA (analysis of variance) was used to test the differences in expression. ns, P > 0.05; *, P < 0.05; **, P < 0.01; ***, P < 0.001.

Acknowledgments

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