FIGURE 5

FIGURE 5

Behavioral analysis of 6 dpf zebrafish larvae treated with orexin 2 receptor agonists in the night assay. Locomotor profiles of animals are expressed as mean actinteg units per 10 min treated with C15454 (A), C19069 (C) and TAK-925 (E) are plotted as function of time. Different phases are shown, i.e. the light phase from 8 p.m. to 9 p.m. (i.e., Evening), the first dark phase from 9 p.m. to 9:20 p.m. (i.e., Dark 1), the second dark phase from 9:20 p.m. to 7 a.m. (i.e., Dark 2), and the light phase from 7 a.m. to 8 a.m. (i.e., Morning). For the sake of clarity, standard deviations are not shown. Total locomotor activity was averaged (±SD) per respective light or dark phase (B–F). For each compound, data were pooled from three or four independent experiments with 7–10 replicate wells per test condition (n = 23–30, except for 75 µM C15454 (n = 20) and 25 µM C15454 (n = 10)). Statistical analysis: two-way ANOVA with Dunnett’s multiple comparison test. Significance levels: **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.