FIGURE 4

FIGURE 4

Behavioral analysis of 6 dpf zebrafish larvae treated with orexin receptor antagonists in the night assay. Locomotor profiles of animals are expressed as mean actinteg units per 10 min treated with suvorexant (A), TCS-1102 (C) and SB-674042 (E) are plotted as function of time. Different phases are shown, i.e. the light phase from 8 p.m. to 9 p.m. (i.e., Evening), the first dark phase from 9 p.m. to 9:20 p.m. (i.e., Dark 1), the second dark phase from 9:20 p.m. to 7 a.m. (i.e., Dark 2), and the light phase from 7 a.m. to 8 a.m. (i.e., Morning). For the sake of clarity, standard deviations are not shown. Total locomotor activity was averaged (±SD) per respective light or dark phase (B–F) For each compound, data were pooled from three independent experiments with 9–10 replicate wells per test condition (n = 27–30, except for 15 µM TCS-1102 (n = 20)). Statistical analysis: two-way ANOVA with Dunnett’s multiple comparison test. Significance levels: *p ≤ 0.05, **p ≤ 0.01.