ZFIN Image: Haynes et al., 2022, Figure 2

Image

Figure Caption

Figure 2

klc4^uw314 mutants have reduced Rohon-Beard peripheral axon branching.

(A–B) Stills taken at approximately 24 hpf from long term light sheet movies of wild type (A) or klc4^uw314 mutant (B) embryo development Scale bar = 150µm. (C) Diagram of a 24 hpf embryo indicating the area over the yolk tube extension imaged for axon analysis, with the standard region used for analysis outlined by the white dashed rectangle. (D–E) Representative examples of HNK-1 stained embryos showing peripheral axon branching in wild type (D) and klc4^uw314 I embryos. (F–G) Quantification of axon branching across three technical replicates in wild type (n=32) and klc4^uw314 mutant (n=32) embryos (F) and DMSO (n=12) and kinesore (n=17) treated embryos (G). Error bars = SEM. Statistical significance was measured by comparing the area under the curve for (F–G) using t-test. For (F), *p=0.0378 for entire graph. For (G), *p=0.0484 for shaded region. (H–I) Representative examples of HNK-1 stained embryos showing peripheral axon branching in DMSO (H) and kinesore treated (I) embryos. Scale bars for D,E,H,I = 50 µm.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife

Figure 2

Figure 2

klc4uw314 mutants have reduced Rohon-Beard peripheral axon branching.

klc4^uw314 mutants have reduced Rohon-Beard peripheral axon branching.