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Fig. 5.
(A) Dorsal (left) and sagittal (right) views of coregistered images of Purkinje cells (PC, aldoca:Gal4;UAS:eGFP-CAAX, cyan) and y334-Gal4 vPPNs (dashed white line). Projections from PCs toward vPPNs (white arrowheads) and to hindbrain (yellow arrowheads). Scale bar, 50 μm. (B) Dorsal (top) and sagittal (bottom) confocal slices from y405-Gal4, UAS:kaede (red), and UAS:gphn-FingR-mCherry (cyan) transgenic larvae. Arrowheads show postsynaptic gephyrin puncta. Inset shows imaging window. Scale bar, 30 μm. (C) Dorsal (top) and sagittal (bottom) views of 3D reconstructions of eurydendroid cell projections (EC; gray) coregistered with y334-Gal4 vPPNs (red). Scale bar, 100 μm. (D) Top: Schematic diagram of ethanol exposure experiment. Bottom, left: Change in swim speed (mm/s) after vibratory stimulus compared to average baseline in control (gray, n = 18) and 300 mM ethanol exposed (red, n = 18) shown as change in speed following vibrational stimuli. Effect size is Cohen’s d. t test, P < 0.001. Bottom, right: Change in swim speed (mm/s) in control (gray, n = 18) and ethanol washout (blue, n = 18) fish. Effect size is Cohen’s d. t test, P = 0.72. (E) Change in swim speed (mm/s) after bath application of GABA (n = 27 larvae per group). Top row shows raw change in speed and bottom row shows 95% CI of mean differences relative to control. ANOVA, P = 0.01. (F) GABAA receptor complex and ICL of γ subunit. (G) Left: Changes in swim speed (mm/s) following vibratory stimuli in y334-Gal4;UAS:ICL-GFP (ICL, red, n = 47) and sibling controls (gray, n = 61). t test, P = 0.007. Right: Changes in swim speed (mm/s) following vibratory stimuli in y334-Gal4;UAS:ICLmut-GFP (ICLmut, red, n = 40) and sibling controls (gray, n = 47). t test, P = 0.91.