Figure 2

Intraperitoneal injection of λ-CGN stimulates a highly selective cellular immune response in zebrafish. (A) The time-dependent infiltrating mean number of total leukocytes in control (PBS) and λ-CGN-injected fish with two different concentrations. (B) Quantification of the progressive mean abdominal edema expansion using the highest (3.5%) λ-CGN concentration 4h after the injection. (C) Representative dot plot showing the gating used to discriminate between lymphocytes [L], neutrophils [N], and monocytes [M] present in the ascites recovered from the PBS (red) or 3.5% λ-CGN-treated (blue) fish 4h post-injection. (D–F) Quantification of data in (C) by cellular type. (Note the resulting inhibition in the monocyte fraction) (n = 21 fish per group) Results were analyzed using an unpaired t-test (p< 0.05) respective to the control value. Error bars indicate SD.