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Fig. 7
a, b Spaw knockdown resulted in failure of the clockwise shift of the posterior border between the left and right heart primordia. a Selected images from a confocal time-lapse recording of a spaw MO-injected Tg(myl7:EGFP-CAAX)ncv536Tg embryo starting at 19–20 hpf (Supplementary Movie 7). Dorsal view (anterior to the top). Scale bar = 50 µm. b Colored, corresponding images to those in a. Cells derived from the left (magenta) and right (green) primordia are color-coded. Cells with ambiguous left-right origins are left uncolored. c–e Quantification of the convergence of the left (L) and right (R) primordia. The anterior portion of the primordia (colored regions in c) were analyzed. Scale bar in c = 50 µm. d Plots of the relative lengths (Lt/L0) of peripheral (1) and inner (2) cell arrays in spaw MO-injected embryos. No significant difference was observed between the left (magenta plots) and right (green plots) cell arrays in both the peripheral and inner regions (two-tailed t-test assuming unequal variances, n = 3 embryos), unlike uninjected embryos (Fig. 5). Means ± s.d. are shown. e Relative length changes of cell arrays (black), cells (blue), and loss of the cell lengths caused by the cell overlap (red) in the peripheral (1) and inner (2) cell arrays in spaw MO-injected embryos. n = 3 embryos. Means ± s.d. are shown. For d, e, corresponding graphs indicating individual data points are shown in Supplementary Fig. 6.