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Fig. 6
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Figure Caption
Fig. 6. S1β shows delayed but stronger proinflammatory activity than the S1WT.
Recombinant S1WT, S1β, or vehicle (−) were injected in the hindbrain ventricle of 2-dpf larvae of Tg(mpx:eGFP) (A), Tg(mfap4:mCherry) (B), Tg(nfkb:eGFP) (C), and WT (D and E). Neutrophil (A) and macrophage (B) recruitment and number and Nfkb activation (C) were analyzed at 6, 12, and 24 hpi by fluorescence microscopy; the transcript levels of the indicated genes were analyzed at 12 hpi by RT-qPCR (D); and caspase-1 activity was determined at 24 hpi using a fluorogenic substrate (E). Each dot represents one individual and the means ± SEM for each group is also shown. RT-qPCR data are depicted as a heatmap in (D) with higher expression shown in darker color. P values were calculated using one-way ANOVA and Tukey multiple range test. *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001.
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